HPLC Column, RP C4 Butyl, Wide Pore, 5um, 4.6mm ID x 250mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4™ Wide Pore is a reversed-phase HPLC stationary phase designed for the separation of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore stationary phase provides enhanced accessibility for larger biomolecules while maintaining the reduced hydrophobic retention characteristics of C4 chemistry. The wide pore architecture makes this phase particularly useful for bioanalytical and biopharmaceutical applications where larger molecules require efficient interaction with the bonded surface.
68504-25P-3M is an HPLC column packed with 5 µm RP C4™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity within the RP C4™ product family, while the 300 Å pore structure improves accessibility and mass transfer for proteins, peptides, enzymes, and other large biomolecules. The 5 µm particles provide low backpressure and robust operation, making the column suitable for routine HPLC systems. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for bioanalytical workflows involving proteins, peptides, enzymes, and other high molecular weight compounds. Compared to more hydrophobic C8 and C18 phases, the C4 bonded phase generally provides lower retention, which can improve recovery and peak shape for larger analytes. The 250 mm format provides maximum resolving power and peak capacity for complex biomolecule separations, method development, and demanding biopharmaceutical analyses.
Key Benefits
RP C4™ Wide Pore stationary phase
USP L26 equivalent chemistry
Butyl (C4) bonded phase
Fully double end capped
Ultra-high purity silica support
300 Å wide pore design
Optimized for proteins and peptides
Lower hydrophobic retention than C8 phases
5 µm particle size
Maximum chromatographic resolution
Typical Applications
Protein analysis
Peptide analysis
Enzyme analysis
Bioanalytical methods
Biomolecule characterization
Pharmaceutical analysis
Biopharmaceutical analysis
Reversed-phase HPLC
Method development
Quality control testing
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4™ Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 4.6 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 1.0–2.0 mL/min
Brand: Cogent
Important Notes
Designed for proteins, peptides, enzymes, and other large biomolecules
300 Å wide pores improve accessibility for larger analytes
Provides less retention than C8 and C18 stationary phases
Suitable for reversed-phase HPLC applications
The 250 mm length provides maximum resolution and peak capacity for challenging biomolecule separations
5 µm particles provide low backpressure and robust operation
Fully double end capped to minimize secondary interactions
Frequently used for bioanalytical and biopharmaceutical applications
Excellent choice for protein and peptide method development
Ideal for biomolecule separations requiring improved pore accessibility